Western Blot:Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Immunoblot analysis Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Homogenization:Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Immunoblot analysis Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
SDS Page:Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Immunoblot analysis Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Membrane:Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Immunoblot analysis Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Pore Size:Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Immunoblot analysis Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
Article Title: H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation
Article Snippet: .. Protein extractions from third instar larval salivary glands (homogenization buffer: 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.2% Triton X-100, 0.2% NP-40, 2 mM Na 3 VO 4 , 1 mM PMSF, 1.5 μg/ml aprotinin) were separated by SDS-PAGE and immunoblotted ( Sambrook and Russell, 2001 ) using the Bio-Rad Mini PROTEAN III system and nitrocellulose membrane (0.2 μm pore size). .. Anti-mouse, anti-chicken or anti-rabbit HRP-conjugated secondary antibody (Bio-Rad; 1:3000) was used to detect primary antibody using a ChemiDoc-It TS2 imager (UVP).
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